b6ea0b654c
* bwa: add page --------- Co-authored-by: CleanMachine1 <78213164+CleanMachine1@users.noreply.github.com> Co-authored-by: K.B.Dharun Krishna <kbdharunkrishna@gmail.com> Co-authored-by: Sebastiaan Speck <12570668+sebastiaanspeck@users.noreply.github.com>
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bwa
Burrows-Wheeler Alignment tool. Short, low-divergent DNA sequences mapper against a large reference genome, such as the human genome. More information: https://github.com/lh3/bwa.
- Index the reference genome:
bwa index {{path/to/reference.fa}}
- Map single-end reads (sequences) to indexed genome using 32 [t]hreads and compress the result to save space:
bwa mem -t 32 {{path/to/reference.fa}} {{path/to/read_single_end.fq.gz}} | gzip > {{path/to/alignment_single_end.sam.gz}}
- Map pair-end reads (sequences) to the indexed genome using 32 [t]hreads and compress the result to save space:
bwa mem -t 32 {{path/to/reference.fa}} {{path/to/read_pair_end_1.fq.gz}} {{path/to/read_pair_end_2.fq.gz}} | gzip > {{path/to/alignment_pair_end.sam.gz}}
- Map pair-end reads (sequences) to the indexed genome using 32 [t]hreads with [M]arking shorter split hits as secondary for output SAM file compatibility in Picard software and compress the result:
bwa mem -M -t 32 {{path/to/reference.fa}} {{path/to/read_pair_end_1.fq.gz}} {{path/to/read_pair_end_2.fq.gz}} | gzip > {{path/to/alignment_pair_end.sam.gz}}
- Map pair-end reads (sequences) to indexed genome using 32 [t]hreads with FASTA/Q [C]omments (e.g. BC:Z:CGTAC) appending to a compressed result:
bwa mem -C -t 32 {{path/to/reference.fa}} {{path/to/read_pair_end_1.fq.gz}} {{path/to/read_pair_end_2.fq.gz}} | gzip > {{path/to/alignment_pair_end.sam.gz}}